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Verifications of mRNA Vaccine DNA Contamination in the World (As of 2025-04-01)
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ResearcherAffiliation, CountryPharma Company# of VialsFirst
reported
MethodsDNA/dose (limit 10ng)DNA/RNA ratio (limit 1/3030)ConcernsPublicationReferences
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McKernan, K.Medicinal Genomics, USPfizer, Modernaa dozen2023-02Electrophoresis (Agilent)2250ng -- 3390ng [1]1/8 -- 1/2Adverse events, Gene integrationReported to FDA
Presented at FDA
Presented at WCH
Reported by Epochtimes

Found gene integration in OvCar3 cancer cells transfected by Kämmerer
Found SV40 in tumors
https://anandamide.substack.com/p/sequencing-of-bivalent-moderna-and
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Fluorometer (Qubit)312ng -- 843ng [1]1/47 -- 1/8
https://x.com/lawrie_dr/status/1708929219797864552
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qPCR/RT-qPCR12ng1/161 -- 1/43
https://x.com/Kevin_McKernan/status/1714032942748705043
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Pfizer, Moderna,
Daiichi-Sankyo
(Japan)
52023-11Fluorometer (Qubit)17.5 -- 61.8ng (after Tritton-X/RNaseA)
https://anandamide.substack.com/p/fluorometry-deep-dive
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qPCR88.8ng (Pfizer)
https://anandamide.substack.com/p/vaccine-targeted-qpcr-of-cancer-cell
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Nitta, T.Tokyo Univ, JapanModerna1 or 22023-03qPCR/RT-qPCR0.1ng [2]1/1,000,000No ProblemNo publication
https://medicalstats.net/med/dna-stdcurves-20230616.html
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Buckhaults, P. J.University of South Carolina, USPfizer, Modernasome2023-07qPCR0.6ng -- 18.7ngAdverse events, Gene integrationPresented in South Caloraina Senate

Presented gene integration to normal human epithelial stem cells
https://x.com/P_J_Buckhaults/status/1684569351998038017
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Pfizer 2020,
Pfizer 2023,
Moderna 2020,
Moderna 2023
42024-04qPCR7.7ng (SV40, Pfizer)
4.5 -- 5.5ng (Neo/Kan, Pfizer)
1.5 -- 9.0ng (ORI, Pfizer)
2.5 -- 18.7ng (Spike, Pfizer)
0.002 -- 0.004ng
(ORI, Moderna 2023)
https://x.com/P_J_Buckhaults/status/1782551861083685223
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König, B.
Kirchner, J. O.
MMD, Germany
Indep., Germany
Pfizer, Moderna42023-09Fluorometer (Qubit)3600ng -- 5340ng1/12 -- 1/7Adverse events, Gene integrationReported to the German Ministry of Health
Reported by local public broadcasting
MDPI peer-reviewed paper
https://x.com/AnwaltUlbrich/status/1704181944815157324
https://x.com/EpochTimesDE/status/1704186543810691393
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Speicher, D. J.

McKernan, K.
University of Guelph, Canada

Medicinal Genomics, US
Pfizer, Moderna272023-10Fluorometer (Qubit)1896ng -- 5100ngAdverse events, Gene integrationPreprint
Presented at WCH
https://osf.io/preprints/osf/mjc97
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qPCR0.22ng -- 2.43ng (Spike)
0.01ng -- 4.27ng (ORI)
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Speicher, D. J.University of Guelph, CanadaPfizer, Moderna
(Australia)
32024-06Fluorometer (Qubit)451ng -- 1420ng (after RNaseA/DNaseI)Adverse events, Gene integrationReported to Therapeutic Goods Administration (TGA)
Under litigation
https://julesonthebeach.substack.com/p/australian-dna-contamination-confirmed?r=1vqgrj&utm_medium=ios&triedRedirect=true
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qPCR6.46ng -- 163.68ng (Spike)
0.54ng -- 12.97ng (ORI)
3.70ng -- 14.69ng (SV40, Pfizer)
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Pfizer (Ireland, incl. XBB.1.5 and JN.1)62025-03qPCR46.16 -- 88.76ng (Spike)
5.59 -- 37.77ng (ORI)
9.13 -- 52.15ng (SV40)
Gene integrationSubstack
https://www.courageoustruth.davidspeicher.com/p/pfizer-comirnaty-jn1-booster-contains
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Raoult, D.Aix-Marseille Univ
(Former Prof), France
Pfizersome2024-11Fluorometer (Qubit)216ng (Avg)
5160ng (Avg,
after Triton-X-100)
Gene integrationPreprint
https://x.com/raoult_didier/status/1857773158775873813
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Kämmerer, U.Univ. Hospital of Würzburg, GermanyPfizer42024-12Fluorometer (Qubit)2712 -- 3683ng
(after Triton-X-100)
32.71 -- 42.09ng
(after Triton-X-100/RNaseA)
Adverse events, Gene integrationPeer-reviewed paper of Science, public health policy and the law
Transfection to HEK293 cells
https://publichealthpolicyjournal.com/biontech-rna-based-covid-19-injections-contain-large-amounts-of-residual-dna-including-an-sv40-promoter-enhancer-sequence/
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Wang, T. J.
Kim, A., Kim, K.
(FDA scientists
helped them)
Centreville High School, US
(executed at FDA White Oak Campus)
Pfizer6
(3 for each of 2 out of 4 lots)
2024-12Fluorometer (Qubit)41.4 -- 109.5ng
(extracted by Monarch Plasmid DNA Miniprep, which includes RNaseA)
Gene integrationPeer-reviewed paper of Journal of High School Science
https://jhss.scholasticahq.com/article/127890-a-rapid-detection-method-of-replication-competent-plasmid-dna-from-covid-19-mrna-vaccines-for-quality-control
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Pekova, S.TILIA LABORATORIES s.r.o., CzechPfizer, Moderna120
(5 ampules for each of 24 lots)
2025-03qPCR10 million -- 1 billion copies/mlGene Integration
Tumorigenesis
An announcement by a Slovak government investigator (member of parliament)
https://www.10letters.org/CzechResearch.pdf
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[1] Multiplied the value by 300 for ul
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[2] From the description of DNA 44x10fg to mRNA 400ng, the calculation for Moderna 1-dose as mRNA 100ug
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世界におけるmRNAワクチンのDNA汚染検証 (2025/04/01版)
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研究者所属、国製造元バイアル数最初の
報告年月
手法接種当りDNA
(上限10ng)
DNA/RNA 比 (上限 1/3030)懸念報告・発表・報道
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McKernan, K.Medicinal Genomics, 米国Pfizer, Moderna十数本2023-02電気泳動 (Agilent)2250ng 〜 3390ng [1]1/8 〜 1/2副反応、
遺伝子組込み
FDAに報告
FDAで発表
WCHで発表
Epochtimes報道

Kämmerer, U. によって処理されたOvCar3癌細胞に遺伝子統合を発見
腫瘍内にSV40を発見
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蛍光光度計 (Qubit)312ng 〜 843ng [1]1/47 〜 1/8
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qPCR/RT-qPCR12ng1/161 〜 1/43
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Pfizer, Moderna,
第一三共
(日本)
52023-12蛍光光度計 (Qubit)17.5 〜 61.8ng (TrittonX/RNaseA処理後)
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qPCR88.8ng (Pfizer)
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新田剛東京大学, 日本Moderna1または22023-03qPCR/RT-qPCR0.1ng [2]1/1,000,000問題無い特に無し
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Buckhaults, P. J.サウスカロライナ大学, 米国Pfizer数本2023-07qPCR0.6ng 〜 18.7ng副反応、
遺伝子組込み
サウスカロライナ州上院で発表

正常なヒト上皮幹細胞への遺伝子組込みを提示
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Pfizer 2020,
Pfizer 2023,
Moderna 2020,
Moderna 2023
42024-04qPCR7.7ng (SV40, Pfizer)
4.5 〜 5.5ng (Neo/Kan, Pfizer)
1.5 〜 9.0ng (ORI, Pfizer)
2.5 〜 18.7ng (Spike, Pfizer)
0.002 〜 0.004ng (ORI, Moderna 2023)
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König, B.
Kirchner, J. O.
MMD, ドイツ
個人, ドイツ
Pfizer72023-09蛍光光度計 (Qubit)3600ng 〜 5340ng1/12 〜 1/7副反応、
遺伝子組込み
ドイツ保険省に報告
地方公共放送で報道
MDPI 査読済論文発表
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Speicher, D. J.

McKernan, K.

ゲルフ大学, カナダ

Medicinal Genomics, 米国
Pfizer, Moderna272023-10蛍光光度計 (Qubit)1896ng 〜 5100ng副反応、
遺伝子組込み
プレプリント
McKernan氏がWCHで発表
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qPCR0.22ng 〜 2.43ng (spike)
0.01ng 〜 4.27ng (ori)
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Speicher, D. J.ゲルフ大学, カナダPfizer, Moderna
(オーストラリア)
32024-06蛍光光度計 (Qubit)451ng 〜 1420ng (RNaseA/DNaseI処理後)副反応、
遺伝子組込み
オーストラリアTGA(治療薬管理局)に報告
訴訟中
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qPCR6.46ng 〜 163.68ng (spike)
0.54ng 〜 12.97ng (ori)
3.70ng 〜 14.69ng (SV40, Pfizer)
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Pfizer (アイルランド, XBB.1.5とJN.1含む)62025-03qPCR46.16 〜 88.76ng (Spike)
5.59 〜 37.77ng (ORI)
9.13 〜 52.15ng (SV40)
遺伝子組込みSubstack
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Raoult, D.エクス=マルセイユ
大学(元教授),
フランス
Pfizer数本2024-11蛍光光度計 (Qubit)216ng (平均)
5160ng
(平均,Triton-X-100処理後)
遺伝子組込みプレプリント
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Kämmerer, U.ヴュルツブルク大学病院, ドイツPfizer42024-12蛍光光度計 (Qubit)2712 〜 3683ng
(Triton-X-100処理後)
32.71 〜 42.09ng
(Triton-X-100/RNaseA処理後)
副反応、
遺伝子組込み
Science, public health policy and the law において査読済論文発表
HEK293細胞への導入
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Wang, T. J.
Kim, A., Kim, K.
(FDAの科学者が
手助け)
センタービル高校,
米国
(FDAホワイトオークキャンパスで実施)
Pfizer6
(4種のロットから2つに各3本)
2024-12蛍光光度計 (Qubit)41.4 〜 109.5ng
(RNaseA を含む Monarch Plasmid DNA Miniprepで抽出)
遺伝子組込みJournal of High School Science において査読済論文発表
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Pekova, S.TILIA LABORATORIES s.r.o., チェコPfizer, Moderna120
(5 ampules for each of 24 lots)
2025-03qPCR1000万 〜 10億コピー/ml遺伝子組込み
腫瘍形成
スロバキア政府の捜査官(国会議員)による発表
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[1] ul 当りの数値に300を乗じた
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[2] mRNA 400ng当りDNA 10x44fgの記述から、モデルナ1dose 100ugとして計算
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